Fluorescent GD2 analog for single-molecule imaging.
Glycoconj J
; 40(2): 247-257, 2023 04.
Article
em En
| MEDLINE
| ID: mdl-36701103
ABSTRACT
Ganglioside GD2 is associated with the proliferation and migration of breast cancer cells. However, the precise role of GD2 is unclear because its tendency to form dynamic and transient domains in cell plasma membranes (PMs), called lipid rafts, makes it difficult to observe. Previously, we developed fluorescent analogs of gangliosides (e.g., GM3 and GM1), which enabled the observation of lipid raft formation for the first time using single-molecule imaging. In this report, we describe the first chemical synthesis of a fluorescent ganglioside, GD2. A biophysical analysis of the synthesized analog revealed its raft-philic character, suggesting its potential to aid single-molecule imaging-based investigations into raft-associated interactions.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imagem Individual de Molécula
/
Gangliosídeos
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article