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Simultaneous sequencing of genetic and epigenetic bases in DNA.
Füllgrabe, Jens; Gosal, Walraj S; Creed, Páidí; Liu, Sidong; Lumby, Casper K; Morley, David J; Ost, Tobias W B; Vilella, Albert J; Yu, Shirong; Bignell, Helen; Burns, Philippa; Charlesworth, Tom; Fu, Beiyuan; Fordham, Howerd; Harding, Nicolas J; Gandelman, Olga; Golder, Paula; Hodson, Christopher; Li, Mengjie; Lila, Marjana; Liu, Yang; Mason, Joanne; Mellad, Jason; Monahan, Jack M; Nentwich, Oliver; Palmer, Alexandra; Steward, Michael; Taipale, Minna; Vandomme, Audrey; San-Bento, Rita Santo; Singhal, Ankita; Vivian, Julia; Wójtowicz, Natalia; Williams, Nathan; Walker, Nicolas J; Wong, Nicola C H; Yalloway, Gary N; Holbrook, Joanna D; Balasubramanian, Shankar.
Afiliação
  • Füllgrabe J; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Gosal WS; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Creed P; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Liu S; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Lumby CK; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Morley DJ; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Ost TWB; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Vilella AJ; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Yu S; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Bignell H; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Burns P; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Charlesworth T; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Fu B; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Fordham H; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Harding NJ; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Gandelman O; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Golder P; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Hodson C; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Li M; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Lila M; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Liu Y; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Mason J; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Mellad J; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Monahan JM; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Nentwich O; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Palmer A; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Steward M; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Taipale M; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Vandomme A; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • San-Bento RS; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Singhal A; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Vivian J; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Wójtowicz N; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Williams N; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Walker NJ; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Wong NCH; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Yalloway GN; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK.
  • Holbrook JD; Cambridge Epigenetix Ltd, The Trinity Building, Chesterford Research Park, Cambridge, UK. Joanna.holbrook@cegx.co.uk.
  • Balasubramanian S; Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK. sb10031@cam.ac.uk.
Nat Biotechnol ; 41(10): 1457-1464, 2023 Oct.
Article em En | MEDLINE | ID: mdl-36747096
ABSTRACT
DNA comprises molecular information stored in genetic and epigenetic bases, both of which are vital to our understanding of biology. Most DNA sequencing approaches address either genetics or epigenetics and thus capture incomplete information. Methods widely used to detect epigenetic DNA bases fail to capture common C-to-T mutations or distinguish 5-methylcytosine from 5-hydroxymethylcytosine. We present a single base-resolution sequencing methodology that sequences complete genetics and the two most common cytosine modifications in a single workflow. DNA is copied and bases are enzymatically converted. Coupled decoding of bases across the original and copy strand provides a phased digital readout. Methods are demonstrated on human genomic DNA and cell-free DNA from a blood sample of a patient with cancer. The approach is accurate, requires low DNA input and has a simple workflow and analysis pipeline. Simultaneous, phased reading of genetic and epigenetic bases provides a more complete picture of the information stored in genomes and has applications throughout biomedicine.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article