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Analysis of the reliability of rapid diagnostic tests for varicella, including breakthrough cases.
Higashimoto, Yuki; Hattori, Fumihiko; Kawamura, Yoshiki; Kozawa, Kei; Hamano, Aoi; Kato, Mizuki; Kato, Sayaka; Hosokawa, Asuka; Enya, Yasuko; Ihira, Masaru; Yoshikawa, Tetsushi.
Afiliação
  • Higashimoto Y; Department of Cellular and Molecular Biology, Fujita Health University School of Medical Sciences, Toyoake, Aichi, Japan.
  • Hattori F; Department of Pediatrics, Kariya Toyota General Hospital, Kariya, Aichi, Japan.
  • Kawamura Y; Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
  • Kozawa K; Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
  • Hamano A; Department of Cellular and Molecular Biology, Fujita Health University School of Medical Sciences, Toyoake, Aichi, Japan.
  • Kato M; Department of Cellular and Molecular Biology, Fujita Health University School of Medical Sciences, Toyoake, Aichi, Japan.
  • Kato S; Department of Cellular and Molecular Biology, Fujita Health University School of Medical Sciences, Toyoake, Aichi, Japan.
  • Hosokawa A; Department of Cellular and Molecular Biology, Fujita Health University School of Medical Sciences, Toyoake, Aichi, Japan.
  • Enya Y; Department of Clinical Science for Biological Monitoring, Fujita Health University School of Medical Sciences, Toyoake, Aichi, Japan.
  • Ihira M; Department of Clinical Science for Biological Monitoring, Fujita Health University School of Medical Sciences, Toyoake, Aichi, Japan.
  • Yoshikawa T; Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
J Med Virol ; 95(2): e28569, 2023 02.
Article em En | MEDLINE | ID: mdl-36762573
In the era of universal varicella vaccination, diagnosis of varicella is challenging, especially for breakthrough cases. We sought to clarify the reliability of direct varicella-zoster virus (VZV) loop-mediated isothermal amplification (LAMP) and DermaQuick® VZV using the immunochromatography technique as rapid diagnostic tests for varicella. In addition, the usefulness of saliva as a sample type for direct LAMP was investigated. Among the 46 enrolled patients with suspected VZV infection, 31 patients (67.3%) were positive for the nucleic acid test based on real-time PCR from skin swab samples. Direct LAMP of skin swabs was positive in 29 (63.0%) of 46 patients. DermaQuick® VZV was positive in 25 (54.3%) of 46 patients. VZV DNA was detected in only 48.4% of oral swabs with the direct LAMP method. With real-time polymerase chain reaction (PCR) as the standard for diagnosing varicella, the sensitivity and specificity of DermaQuick® VZV were 80.7% and 100%, respectively. The sensitivity and specificity of direct LAMP from skin swabs were 93.6% and 100%, respectively. The sensitivity and specificity of real-time PCR for DNA extracted from oral swabs were 74.2% and 93.3%, respectively. Thus, oral swab samples are not suitable for breakthrough varicella diagnosis. Although DermaQuick® VZV is considered the most convenient point-of-care test for varicella, its sensitivity and specificity were lower than those of direct VZV LAMP.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Varicela / Herpes Zoster Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Varicela / Herpes Zoster Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article