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Timosaponin BII inhibits TGF-ß mediated epithelial-mesenchymal transition through Smad-dependent pathway during pulmonary fibrosis.
Ding, Dali; Shen, Xuebin; Yu, Lizhen; Zheng, Yueyue; Liu, Yao; Wang, Wei; Liu, Li; Zhao, Zitong; Nian, Sihui; Liu, Limin.
Afiliação
  • Ding D; School of Pharmacy, Wannan Medical College, Wuhu, China.
  • Shen X; Pharmacy Department, The PLA Navy Anqing Hospital, Anqing, China.
  • Yu L; School of Pharmacy, Wannan Medical College, Wuhu, China.
  • Zheng Y; Institute of Modern Chinese Medicine, Wannan Medical College, Wuhu, China.
  • Liu Y; School of Pharmacy, Wannan Medical College, Wuhu, China.
  • Wang W; Institute of Modern Chinese Medicine, Wannan Medical College, Wuhu, China.
  • Liu L; Anhui Provincial Engineering Laboratory for Screening and Re-evaluation of Active Compounds of Herbal Medicines in Southern Anhui, Wannan Medical College, Wuhu, China.
  • Zhao Z; School of Pharmacy, Wannan Medical College, Wuhu, China.
  • Nian S; School of Pharmacy, Wannan Medical College, Wuhu, China.
  • Liu L; School of Pharmacy, Wannan Medical College, Wuhu, China.
Phytother Res ; 37(7): 2787-2799, 2023 Jul.
Article em En | MEDLINE | ID: mdl-36807664
Pulmonary fibrosis (PF) is a progressive and fatal interstitial lung disease with limited therapeutic options at present, and epithelial-mesenchymal transition (EMT) is recognized as a major cause of lung fibrosis. Our previous work has confirmed that total extract of Anemarrhena asphodeloides Bunge [Asparagaceae] exerted the effect of anti-PF. As a main constituent of Anemarrhena asphodeloides Bunge [Asparagaceae], the effect of timosaponin BII (TS BII) on drug-induced EMT process in PF animals and alveolar epithelial cells remains unknown. In this study, we evaluated the effect of TS BII on bleomycin (BLM)-induced PF. The results showed that TS BII could restore the structure of lung architecture and MMP-9/TIMP-1 balance in fibrotic rat lung and inhibit collagen deposition. Moreover, we found that TS BII could reverse the abnormal expression of TGF-ß1 and EMT-related marker proteins including E-cadherin, vimentin, and α-SMA. Besides, aberrant TGF-ß1 expression and phosphorylation of Smad2 and Smad3 in BLM-induced animal model and TGF-ß1-induced cell model were downregulated by TS BII treatment, indicating that EMT in fibrosis was suppressed by inhibition of TGF-ß/Smad pathway both in vivo and in vitro. In summary, our study suggested that TS BII could be a promising candidate for PF treatment.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fibrose Pulmonar Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fibrose Pulmonar Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article