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Species-Level Characterization of the Microbiome in Breast Tissues with Different Malignancy and Hormone-Receptor Statuses Using Nanopore Sequencing.
Luo, Lan; Fu, Aisi; Shi, Manman; Hu, Jiawei; Kong, Deguang; Liu, Tiangang; Yuan, Jingping; Sun, Shengrong; Chen, Chuang.
Afiliação
  • Luo L; Department of Thyroid and Breast Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.
  • Fu A; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education and Wuhan University School of Pharmaceutical Sciences, Wuhan 430060, China.
  • Shi M; Department of Thyroid and Breast Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.
  • Hu J; Department of Thyroid and Breast Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.
  • Kong D; Department of Thyroid and Breast Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.
  • Liu T; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education and Wuhan University School of Pharmaceutical Sciences, Wuhan 430060, China.
  • Yuan J; Department of Pathology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
  • Sun S; Department of Thyroid and Breast Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.
  • Chen C; Department of Thyroid and Breast Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.
J Pers Med ; 13(2)2023 Jan 19.
Article em En | MEDLINE | ID: mdl-36836409
Unambiguous evidence indicates that microbes are closely linked to various human diseases, including cancer. Most prior work investigating the microbiome of breast tissue describes an association between compositional differences of microbial species in benign and malignant tissues, but few studies have examined the relative abundance of microbial communities within human breast tissue at the species level. In this work, a total of 44 breast tissue samples including benign and malignant tissues with adjacent normal breast tissue pairs were collected, and Oxford Nanopore long-read sequencing was employed to assess breast tissue microbial signatures. Nearly 900 bacterial species were detected from the four dominant phyla: Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. The bacteria with the highest abundance in all breast tissues was Ralstonia pickettii, and its relative abundance increased with decreasing malignancy. We further examined the breast-tissue microbiome composition with different hormone-receptor statuses, and the relative abundance of the genus Pseudomonas increased most significantly in breast tissues. Our study provides a rationale for exploring microbiomes associated with breast carcinogenesis and cancer development. Further large-cohort investigation of the breast microbiome is necessary to characterize a microbial risk signature and develop potential microbial-based prevention therapies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article