A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells.
Chromosome Res
; 31(1): 11, 2023 02 26.
Article
em En
| MEDLINE
| ID: mdl-36842155
ABSTRACT
Alterations of human karyotype caused by chromosomal rearrangements are often associated with considerable phenotypic effects. Studying molecular mechanisms underlying these effects requires an efficient and scalable experimental model. Here, we propose a Cre-LoxP-based approach for the generation of combinatorial diversity of chromosomal rearrangements. We demonstrate that using the developed system, both intra- and inter-chromosomal rearrangements can be induced in the human haploid HAP1 cells, although the latter is significantly less effective. The obtained genetically modified HAP1 cell line can be used to dissect genomic effects associated with intra-chromosomal structural variations.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
/
Rearranjo Gênico
/
Cromossomos
Limite:
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article