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Interstrand crosslinking of homologous repair template DNA enhances gene editing in human cells.
Ghasemi, Hannah I; Bacal, Julien; Yoon, Amanda C; Tavasoli, Katherine U; Cruz, Carmen; Vu, Jonathan T; Gardner, Brooke M; Richardson, Chris D.
Afiliação
  • Ghasemi HI; Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA.
  • Bacal J; Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA.
  • Yoon AC; Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA.
  • Tavasoli KU; Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA.
  • Cruz C; Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA.
  • Vu JT; Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA.
  • Gardner BM; Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA.
  • Richardson CD; Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA. chrisrichardson@ucsb.edu.
Nat Biotechnol ; 41(10): 1398-1404, 2023 Oct.
Article em En | MEDLINE | ID: mdl-36849829
ABSTRACT
We describe a strategy to boost the efficiency of gene editing via homology-directed repair (HDR) by covalently modifying the template DNA with interstrand crosslinks. Crosslinked templates (xHDRTs) increase Cas9-mediated editing efficiencies by up to fivefold in K562, HEK293T, U2OS, iPS and primary T cells. Increased editing from xHDRTs is driven by events on the template molecule and requires ataxia telangiectasia and Rad3-related (ATR) kinase and components of the Fanconi anemia pathway.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article