Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices.
STAR Protoc
; 4(1): 102041, 2023 03 17.
Article
em En
| MEDLINE
| ID: mdl-36853668
ABSTRACT
Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.
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Coleções:
01-internacional
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MEDLINE
Assunto principal:
Encéfalo
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article