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TiO2 nanoparticles' library toxicity (UV and non-UV exposure) - High-throughput in vivo transcriptomics reveals mechanisms.
Gomes, Susana I L; Roca, Carlos P; Pokhrel, Suman; Mädler, Lutz; Scott-Fordsmand, Janeck J; Amorim, Mónica J B.
Afiliação
  • Gomes SIL; Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal.
  • Roca CP; Department of Ecoscience, Aarhus University, C.F. Møllers Alle 4, DK-8000, Aarhus, Denmark.
  • Pokhrel S; Department of Production Engineering, University of Bremen, Badgasteiner Str. 1, 28359 Bremen, Germany; Leibniz Institute for Materials Engineering IWT, Badgasteiner Str. 3, 28359 Bremen, Germany.
  • Mädler L; Department of Production Engineering, University of Bremen, Badgasteiner Str. 1, 28359 Bremen, Germany; Leibniz Institute for Materials Engineering IWT, Badgasteiner Str. 3, 28359 Bremen, Germany.
  • Scott-Fordsmand JJ; Department of Ecoscience, Aarhus University, C.F. Møllers Alle 4, DK-8000, Aarhus, Denmark.
  • Amorim MJB; Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address: mjamorim@ua.pt.
NanoImpact ; 30: 100458, 2023 04.
Article em En | MEDLINE | ID: mdl-36858316
The hazards of nanomaterials/nanoparticles (NMs/NPs) are mostly assessed using individual NMs, and a more systematic approach, using many NMs, is needed to evaluate its risks in the environment. Libraries of NMs, with a range of identified different but related characters/descriptors allow the comparison of effects across many NMs. The effects of a custom designed Fe-doped TiO2 NMs library containing 11 NMs was assessed on the soil model Enchytraeus crypticus (Oligochaeta), both with and without UV (standard fluorescent) radiation. Effects were analyzed at organism (phenotypic, survival and reproduction) and gene expression level (transcriptomics, high-throughput 4x44K microarray) to understand the underlying mechanisms. A total of 48 microarrays (20 test conditions) were done plus controls (UV and non-UV). Unique mechanisms induced by TiO2 NPs exposure included the impairment in RNA processing for TiO2_10nm, or deregulated apoptosis for 2%FeTiO2_10nm. Strikingly apparent was the size dependent effects such as induction of reproductive effects via smaller TiO2 NPs (≤12 nm) - embryo interaction, while larger particles (27 nm) caused reproductive effects through different mechanisms. Also, phagocytosis was affected by 12 and 27 nm NPs, but not by ≤11 nm. The organism level study shows the integrated response, i.e. the result after a cascade of events. While uni-cell models offer key mechanistic information, we here deliver a combined biological system level (phenotype and genotype), seldom available, especially for environmental models.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nanopartículas Metálicas / Transcriptoma Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nanopartículas Metálicas / Transcriptoma Idioma: En Ano de publicação: 2023 Tipo de documento: Article