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Evaluation of Slowfade Diamond as a buffer for STORM microscopy.
Boukhatem, Hadjer; Durel, Beatrice; Raimbault, Manon; Laurent, Audrey; Olivier, Nicolas.
Afiliação
  • Boukhatem H; Laboratory for Optics and Biosciences (LOB), CNRS, INSERM, École polytechnique, Institut Polytechnique de Paris, 91120 Palaiseau, France.
  • Durel B; Cell Imaging Platform, Structure Fédérative de Recherche Necker, INSERM US24, CNRS UMS3633, Paris, F-75015, France.
  • Raimbault M; Cell Imaging Platform, Structure Fédérative de Recherche Necker, INSERM US24, CNRS UMS3633, Paris, F-75015, France.
  • Laurent A; Université de Paris, Institut-Necker-Enfants-Malades, Inserm, CNRS, Paris, France.
  • Olivier N; École Doctorale BioSPC 562, Université de Paris, Paris, France.
Biomed Opt Express ; 14(2): 550-558, 2023 Feb 01.
Article em En | MEDLINE | ID: mdl-36874488
ABSTRACT
We study the potential of the commercial mounting medium Slowfade diamond as a buffer for STORM microscopy. We show that although it does not work with the popular far-red dyes typically used for STORM imaging, such as Alexa Fluor 647, it performs really well with a wide variety of green-excited dyes such as Alexa Fluor 532, Alexa Fluor 555 or CF 568. Moreover, imaging can be performed several months after the samples are mounted in this environment and kept in the fridge, providing a convenient way to preserve samples for STORM imaging, as well as to keep calibration samples, for example for metrology or teaching in particular in imaging facilities.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article