A, aqueous phase; AMP, Amphotericin-B; ANOVA, Analysis of variance; Amphotericin-B; Atomic force microscopy (AFM); BCS, Biopharmaceutical Classification System; BLF, Bovine Lactoferrin; CD14, Cluster of differentiation 14; CK, Creatine kinase; Candida albicans; Confocal laser scanning microscopy (CLSM); DLS, dynamic light scattering; DMSO, dimethyl sulfoxide; DSC, Differential scanning calorimetry; Draize test; EDC, ethyl-3-(3-dimethyl aminopropyl) carbodiimide; EE%, Entrapment efficiency; FT-IR, Fourier transform infrared; FT-IR, Fourier-transform infrared spectroscopy; GRAS, Generally recognized as a safe; HCE-2, human corneal epithelial cells; J, steady-state flux; Kp, permeability coefficient; LPS, Lipopolysaccharide; Lactoferrin; MIC, minimum inhibitory concentration; NCCLS, National Committee for Clinical Laboratory Standards; NHS, N-hydroxysuccinimide; NPs, nanoparticles; Nanoparticles; O, organic phase; P188, Kolliphor®P188; P407, Poloxamer 407; PBS, Phosphate buffered saline solution; PDI, Polydispersity index; PEG, polyethylene glycol; PEI, poly-ethylene imine; PLGA, Poly (lactic-co-glycolic acid); PS, Particle size; Q24, amount penetrated after 24 h; QR, Quantity retained; REC, rules of the Study Ethics Committee; SD, Standard deviations; SE, Standard error; SEM, Scanning electron microscope; TEM, Transmission electron microscopy; Triblock polymers PLGA-PEG-PEI; ZP, Zeta potential.