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Illuminating cellular and extracellular vesicle-mediated communication via a split-Nanoluc reporter in vitro and in vivo.
van Solinge, Thomas S; Mahjoum, Shadi; Ughetto, Stefano; Sammarco, Alessandro; Broekman, Marike L D; Breakefield, Xandra O; O'Brien, Killian P.
Afiliação
  • van Solinge TS; Molecular Neurogenetics Unit, Department of Neurology and Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
  • Mahjoum S; Department of Neurosurgery, Leiden University Medical Center, Leiden, the Netherlands.
  • Ughetto S; Molecular Neurogenetics Unit, Department of Neurology and Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
  • Sammarco A; Molecular Neurogenetics Unit, Department of Neurology and Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
  • Broekman MLD; Department of Oncology, University of Turin, Candiolo, Italy.
  • Breakefield XO; Molecular Neurogenetics Unit, Department of Neurology and Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
  • O'Brien KP; Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA.
Cell Rep Methods ; 3(2): 100412, 2023 02 27.
Article em En | MEDLINE | ID: mdl-36936071
ABSTRACT
Tools to effectively demonstrate and quantify functional delivery in cellular communication have been lacking. This study reports the use of a fluorescently labeled split Nanoluc reporter system to demonstrate and quantify functional transfer between cells in vitro and in a subcutaneous tumor mouse model. Our construct allows monitoring of direct, indirect, and specifically extracellular vesicle-mediated functional communication.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vesículas Extracelulares Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vesículas Extracelulares Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article