Expression of epithelial-mesenchymal transition-associated proteins and proliferating cell nuclear antigen in dihydropyridine-induced gingival overgrowth fibroblasts: A preliminary study.
J Dent Sci
; 18(2): 551-559, 2023 Apr.
Article
em En
| MEDLINE
| ID: mdl-37021214
ABSTRACT
Background/purpose:
The clinical features of dihydropyridine-induced gingival overgrowth (DIGO), including extracellular matrix accumulation and cell hyperplasia, are regulated by inflammatory factors (e.g., Interleukin-1ß [IL-1ß]) in combination with calcium channel blockers (e.g., nifedipine [Nif]). We speculated that IL-1ß and Nif (IL-1ß/Nif) may be the main factor modulating the proliferative potential and turnover of fibroblasts in DIGO. Materials andmethods:
We cultured four DIGO fibroblast strains and analysed the possible effects of IL-1ß/Nif treatments on epithelial-mesenchymal transition (EMT)-associated proteins. We developed short hairpin ribonucleic acids (shRNAs) and used them to explore the role of IL-1ß/Nif in regulating proliferating cell nuclear antigen (PCNA) levels in DIGO tissues.Results:
Our results revealed that compared with control cells, DIGO cells stimulated with IL-1ß/Nif had higher levels of the EMT-associated proteins Snail, Slug, and Twist. Moreover, both drugs enhanced androgen receptor (AR), Slug, and PCNA expression.Conclusion:
Taken together, our data indicate that proinflammatory cytokines in combination with calcium channel blockers can regulate the expression of EMT-associated proteins and increase the proliferative potential of DIGO fibroblasts.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article