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Chemical labeling achieves 8-oxo-7,8-dihydroguanine mapping in the microRNA transcriptome.
Fan, Changjiang; Meng, Xinyue; Yang, Wei; Wang, Peiyan; Chang, Wenguang; Li, Peifeng; Wang, Jianxun.
Afiliação
  • Fan C; Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao Medical College, Qingdao University, Qingdao 266021, Shandong, P. R. China. cjfan@qdu.edu.cn.
  • Meng X; School of Basic Medicine, College of Medicine, Qingdao University, Qingdao 266071, Shandong, P. R. China. wangjx@qdu.edu.cn.
  • Yang W; Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao Medical College, Qingdao University, Qingdao 266021, Shandong, P. R. China. cjfan@qdu.edu.cn.
  • Wang P; School of Basic Medicine, College of Medicine, Qingdao University, Qingdao 266071, Shandong, P. R. China. wangjx@qdu.edu.cn.
  • Chang W; Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao Medical College, Qingdao University, Qingdao 266021, Shandong, P. R. China. cjfan@qdu.edu.cn.
  • Li P; School of Basic Medicine, College of Medicine, Qingdao University, Qingdao 266071, Shandong, P. R. China. wangjx@qdu.edu.cn.
  • Wang J; Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao Medical College, Qingdao University, Qingdao 266021, Shandong, P. R. China. cjfan@qdu.edu.cn.
Chem Commun (Camb) ; 59(34): 5106-5109, 2023 Apr 25.
Article em En | MEDLINE | ID: mdl-37039083
ABSTRACT
A labeling chemistry-based methodology, APSC-8-oxoGua-seq, is developed to sequence 8-oxoGua in the microRNA transcriptome. N-(3-Azidopropyl)-spermine-5-carboxamide (APSC) is designed for the selective labeling of 8-oxoGua, where its azide facilitates the conjugation of a cleavable linker via the click reaction, achieving 8-oxoGua pull-down and sequencing. Using APSC-8-oxoGua-seq, 8-oxoGua can be identified at single-base resolution.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: MicroRNAs Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: MicroRNAs Idioma: En Ano de publicação: 2023 Tipo de documento: Article