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Polymorphism and expression level of the FADS3 gene and associated with the growth traits in Hu sheep.
Zeng, Xiwen; Wang, Weimin; Zhang, Deyin; Li, Xiaolong; Zhang, Yukun; Zhao, Yuan; Zhao, Liming; Wang, Jianghui; Xu, Dan; Cheng, Jiangbo; Li, Wenxin; Zhou, Bubo; Lin, Changchun; Yang, Xiaobin; Zhai, Rui; Ma, Zongwu; Liu, Jia; Cui, Panpan; Weng, Xiuxiu; Wu, Weiwei; Zhang, Xiaoxue; Zheng, Wenxin.
Afiliação
  • Zeng X; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
  • Wang W; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
  • Zhang D; The State Key Laboratory of Grassland Agroecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu, China.
  • Li X; The State Key Laboratory of Grassland Agroecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu, China.
  • Zhang Y; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
  • Zhao Y; The State Key Laboratory of Grassland Agroecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu, China.
  • Zhao L; The State Key Laboratory of Grassland Agroecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu, China.
  • Wang J; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
  • Xu D; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
  • Cheng J; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
  • Li W; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
  • Zhou B; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
  • Lin C; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
  • Yang X; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
  • Zhai R; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
  • Ma Z; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
  • Liu J; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
  • Cui P; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
  • Weng X; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
  • Wu W; The State Key Laboratory of Grassland Agroecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu, China.
  • Zhang X; Institute of Animal Science, Xinjiang Academy of Animal Sciences, Urumqi, Xinjiang, China.
  • Zheng W; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
Anim Biotechnol ; 34(9): 4793-4802, 2023 Dec.
Article em En | MEDLINE | ID: mdl-37040177
ABSTRACT
Growth traits are the economically important traits of sheep, and screening for genes related to growth and development is helpful for the genetic improvement of ovine growth traits. The fatty acid desaturase 3 (FADS3) is one of the important genes affecting the synthesis and accumulation of polyunsaturated fatty acids in animals. In this study, the expression levels of the FADS3 gene and polymorphism of the FADS3 gene associated with growth traits in Hu sheep were detected using quantitative real-time PCR (qRT-PCR), Sanger sequencing, and KAspar assay. The result showed that the expression levels of the FADS3 gene were widely expressed in all tissues, and the expression level of FADS3 in the lung was significantly higher than in other tissues (p < .05). Then, the polymorphism locus g. 2918 A > C was detected in intron 2 of the FADS3 gene, and associated analysis showed that the mutation in the FADS3 gene was associated significantly with growth traits (including body weight, body height, body length, and chest circumference, p < .05). Therefore, individuals with AA genotype showed significantly better growth traits than those with CC genotype, and FADS3 gene could be a candidate gene for improving growth traits in Hu sheep.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polimorfismo Genético / Ácidos Graxos Insaturados Tipo de estudo: Risk_factors_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polimorfismo Genético / Ácidos Graxos Insaturados Tipo de estudo: Risk_factors_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article