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Role of Orai3-Mediated Store-Operated Calcium Entry in Radiation-Induced Brain Microvascular Endothelial Cell Injury.
Wu, Qibing; Fang, Yang; Huang, Xiaoyu; Zheng, Fan; Ma, Shaobo; Zhang, Xinchen; Han, Tingting; Gao, Huiwen; Shen, Bing.
Afiliação
  • Wu Q; Department of Radiotherapy, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
  • Fang Y; School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China.
  • Huang X; Department of Radiotherapy, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
  • Zheng F; School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China.
  • Ma S; School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China.
  • Zhang X; Department of Radiotherapy, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
  • Han T; Department of Radiotherapy, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
  • Gao H; School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China.
  • Shen B; School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China.
Int J Mol Sci ; 24(7)2023 Apr 06.
Article em En | MEDLINE | ID: mdl-37047790
Radiation-induced brain injury is a serious complication with complex pathogenesis that may accompany radiotherapy of head and neck tumors. Although studies have shown that calcium (Ca2+) signaling may be involved in the occurrence and development of radiation-induced brain injury, the underlying molecular mechanisms are not well understood. In this study, we used real-time quantitative polymerase chain reaction and Western blotting assays to verify our previous finding using next-generation sequencing that the mRNA and protein expression levels of Orai3 in rat brain microvascular endothelial cells (rBMECs) increased after X-ray irradiation. We next explored the role of Orai3 and Orai3-mediated store-operated Ca2+ entry (SOCE) in radiation-induced brain injury. Primary cultured rBMECs derived from wild-type and Orai3 knockout (Orai3(-/-)) Sprague-Dawley rats were used for in vitro experiments. Orai3-mediated SOCE was significantly increased in rBMECs after X-ray irradiation. However, X-ray irradiation-induced SOCE increase was markedly reduced in Orai3 knockout rBMECs, and the percentage of BTP2 (a nonselective inhibitor of Orai channels)-inhibited SOCE was significantly decreased in Orai3 knockout rBMECs. Functional studies indicated that X-ray irradiation decreased rBMEC proliferation, migration, and tube formation (a model for assessing angiogenesis) but increased rBMEC apoptosis, all of which were ameliorated by BTP2. In addition, occurrences of all four functional deficits were suppressed in X-ray irradiation-exposed rBMECs derived from Orai3(-/-) rats. Cerebrovascular damage caused by whole-brain X-ray irradiation was much less in Orai3(-/-) rats than in wild-type rats. These findings provide evidence that Orai3-mediated SOCE plays an important role in radiation-induced rBMEC damage and brain injury and suggest that Orai3 may warrant development as a potential therapeutic target for reducing or preventing radiation-induced brain injury.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lesões Encefálicas / Canais de Cálcio / Células Endoteliais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lesões Encefálicas / Canais de Cálcio / Células Endoteliais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article