Changes in cytochrome P450 molecular species in rat liver in chloroform intoxication.
Biochem Pharmacol
; 35(9): 1555-60, 1986 May 01.
Article
em En
| MEDLINE
| ID: mdl-3707616
ABSTRACT
The effect of CHCl3 on the composition of hepatic microsomal cytochrome P450 species was compared with that of CCl4 in rats pretreated with phenobarbital (PB) or 3-methylcholanthrene (3MC). The administration of CHCl3 hardly affected cytochrome P450 content in non-treated rat liver, but caused a similar degree of depletion in the content as observed after CCl4 administration in PB-pretreated rats. In the pretreatment with 3MC, the administration of CHCl3 brought about a marked decrease in the content to 24% of control after 12 hr, while CCl4 reduced the content only to one-half of control. It was demonstrated by SDS-polyacrylamide gel electrophoresis and Whatman DE-52 anion-exchange chromatography that 3MC-induced P450 species decreased with CHCl3, while it was affected little by CCl4 treatment. The activity of benzo[a]pyrene hydroxylase was altered together with the change in the content of cytochrome P450 species. The administration of CHCl3 to PB-pretreated rats caused the depletion in PB-induced P450. These findings indicate that cytochrome P450 species induced with 3MC as well as PB are highly susceptible to CHCl3 intoxication, whereas the administration of CCl4 depletes the PB-induced species without affecting the 3MC-induced species.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Clorofórmio
/
Sistema Enzimático do Citocromo P-450
/
Fígado
Limite:
Animals
Idioma:
En
Ano de publicação:
1986
Tipo de documento:
Article