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Whole-mount staining of mouse colorectal cancer organoids and fibroblast-organoid co-cultures.
Martinez-Ordoñez, Anxo; Cid-Diaz, Tania; Duran, Angeles; Han, Qixiu; Moscat, Jorge; Diaz-Meco, Maria T.
Afiliação
  • Martinez-Ordoñez A; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA. Electronic address: amo4002@med.cornell.edu.
  • Cid-Diaz T; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA.
  • Duran A; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA.
  • Han Q; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA.
  • Moscat J; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA.
  • Diaz-Meco MT; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA. Electronic address: mtd4001@med.cornell.edu.
STAR Protoc ; 4(2): 102243, 2023 Apr 20.
Article em En | MEDLINE | ID: mdl-37083323
ABSTRACT
Imaging organoid culture provides an excellent tool for studying complex diseases such as cancer. However, retaining the morphology of intact organoids for immunolabeling has been challenging. Here, we describe a protocol for immunofluorescence staining in intact colorectal cancer organoids derived from mice. We also describe additional steps for co-culture with mouse fibroblasts to enable the study of interactions with other cellular components of the tissue microenvironment. For complete details on the use and execution of this protocol, please refer to Martinez-Ordoñez et al. (2023).1.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article