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SERS assisted sandwich immunoassay platforms for ultrasensitive and selective detection of human Thyroglobulin.
Spaziani, S; Quero, G; Managò, S; Zito, G; Terracciano, D; Macchia, P E; Galeotti, F; Pisco, M; De Luca, A C; Cusano, A.
Afiliação
  • Spaziani S; Optoelectronic Division-Engineering Department, University of Sannio, 82100, Benevento, Italy; Centro Regionale Information Communication Technology (CeRICT Scrl), 82100, Benevento, Italy.
  • Quero G; Optoelectronic Division-Engineering Department, University of Sannio, 82100, Benevento, Italy; Centro Regionale Information Communication Technology (CeRICT Scrl), 82100, Benevento, Italy.
  • Managò S; Institute for Experimental Endocrinology and Oncology "Gaetano Salvatore" (IEOS), Second Unit, National Research Council, 80131, Napoli, Italy.
  • Zito G; Institute of Applied Sciences & Intelligent Systems (ISASI), National Research Council, Naples Unit, 80131, Napoli, Italy.
  • Terracciano D; Dipartimento di Medicina Clinica e Chirurgia, Scuola di Medicina, Università degli Studi di Napoli Federico II, Napoli, 80131, Italy.
  • Macchia PE; Dipartimento di Scienze Mediche Traslazionali, Scuola di Medicina, Università degli Studi di Napoli Federico II, Napoli, 80131, Italy.
  • Galeotti F; Istituto di Scienze e Tecnologie Chimiche "G. Natta" (SCITEC), National Research Council, 20133, Milano, Italy.
  • Pisco M; Optoelectronic Division-Engineering Department, University of Sannio, 82100, Benevento, Italy; Centro Regionale Information Communication Technology (CeRICT Scrl), 82100, Benevento, Italy. Electronic address: pisco@unisannio.it.
  • De Luca AC; Institute for Experimental Endocrinology and Oncology "Gaetano Salvatore" (IEOS), Second Unit, National Research Council, 80131, Napoli, Italy. Electronic address: annachiara.deluca@cnr.it.
  • Cusano A; Optoelectronic Division-Engineering Department, University of Sannio, 82100, Benevento, Italy; Centro Regionale Information Communication Technology (CeRICT Scrl), 82100, Benevento, Italy.
Biosens Bioelectron ; 233: 115322, 2023 Aug 01.
Article em En | MEDLINE | ID: mdl-37100718
We developed an immunoassay platform for the detection of human Thyroglobulin (Tg) to be integrated with fine-needle aspiration biopsy for early detection of lymph node metastases in thyroid cancer patients. The sensing platform detects Tg by a sandwich immunoassay involving a self-assembled surface-enhanced Raman scattering (SERS) substrate assisted by functionalized gold nanoparticles that provide additional Raman signal amplification and improved molecular specificity. Specifically, the SERS-active substrates were functionalized with Tg Capture antibodies and fabricated either on-chip or on optical fiber tips by nanosphere lithography. Gold nanoparticles were functionalized with Detection antibodies and conjugated with 4-mercaptobenzoic acid, which serves as a Raman reporter. The sandwich assay platform was validated in the planar configuration and a detection limit as low as 7 pg/mL was successfully achieved. Careful morphological examination of the SERS substrates before and after Tg measurements further assessed the effective capture of nanoparticles and correlated the average nanoparticle coverage with the Tg concentration obtained by SERS measurements. The sandwich assay was successfully demonstrated on washout fluids of fine needle aspiration biopsies from cancer patients and confirmed the high specificity of the proposed methodology when complex biological matrices are considered. Finally, SERS optrodes were fabricated and successfully used to detect Tg concentration by applying the same bio-recognition strategy and Raman interrogation through an optical fiber. This opens the possibility of transferring the Tg detection approach to the optical fiber tip to develop point-of-care platforms that can be directly integrated into fine needle aspiration biopsies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Nanopartículas Metálicas Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Nanopartículas Metálicas Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article