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Ethanolic Extract of Artemisia vulgaris Leaf Promotes Apoptotic Cell Death in Non-Small-Cell Lung Carcinoma A549 Cells through Inhibition of the Wnt Signaling Pathway.
Tiwari, Rohit Kumar; Ahmad, Afza; Khan, Ahamad Faiz; Al-Keridis, Lamya Ahmed; Saeed, Mohd; Alshammari, Nawaf; Alabdallah, Nadiyah M; Ansari, Irfan Ahmad; Mujeeb, Farina.
Afiliação
  • Tiwari RK; Department of Biosciences, Integral University, Lucknow 226026, Uttar Pradesh, India.
  • Ahmad A; Department of Biosciences, Integral University, Lucknow 226026, Uttar Pradesh, India.
  • Khan AF; Department of Bioengineering, Integral University, Lucknow 226026, Uttar Pradesh, India.
  • Al-Keridis LA; Biology Department, Faculty of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
  • Saeed M; Department of Biology, College of Sciences, University of Hail, P.O. Box 2440, Hail 34464, Saudi Arabia.
  • Alshammari N; Department of Biology, College of Sciences, University of Hail, P.O. Box 2440, Hail 34464, Saudi Arabia.
  • Alabdallah NM; Department of Biology, College of Science, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia.
  • Ansari IA; Basic & Applied Scientific Research Centre, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia.
  • Mujeeb F; Department of Biosciences, Integral University, Lucknow 226026, Uttar Pradesh, India.
Metabolites ; 13(4)2023 Mar 27.
Article em En | MEDLINE | ID: mdl-37110139
ABSTRACT
The Wnt signaling pathway is reported to be associated with lung cancer progression, metastasis and drug resistance, and thus it is an important therapeutic target for lung cancer. Plants have been shown as reservoirs of multiple potential anticancer agents. In the present investigation, the ethanolic leaf extract of Artemisia vulgaris (AvL-EtOH) was initially analyzed by means of gas chromatography-mass spectrometry (GC-MS) to identify the important phytochemical constituents. The GC-MS analysis of AvL-EtOH exhibited 48 peaks of various secondary metabolites such as terpenoids, flavonoids, carbohydrates, coumarins, amino acids, steroids, proteins, phytosterols, and diterpenes. It was found that the treatment with increasing doses of AvL-EtOH suppressed the proliferation and migration of lung cancer cells. Furthermore, AvL-EtOH induced prominent nuclear alteration along with a reduction in mitochondrial membrane potential and increased ROS (reactive oxygen species) generation in lung cancer cells. Moreover, AvL-EtOH-treated cells exhibited increased apoptosis, demonstrated by the activation of caspase cascade. AvL-EtOH also induced downregulation of Wnt3 and ß-catenin expression along with cell cycle protein cyclin D1. Thus, the results of our study elucidated the potential of bioactive components of Artemisia vulgaris in the therapeutic management of lung cancer cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article