CRISPR/Cas9-mediated genome editing of gustatory receptor NlugGr23a causes male sterility in the brown planthopper Nilaparvata lugens.
Int J Biol Macromol
; 241: 124612, 2023 Jun 30.
Article
em En
| MEDLINE
| ID: mdl-37119891
ABSTRACT
Gustatory receptors (Grs) have an essential role in chemical recognition so as to evaluate food quality. Insect Grs also participate in non-gustatory functions, such as olfaction, temperature sensing, and mating. In this study, we knocked out NlugGr23a, a putative fecundity-related Gr, using the CRISPR/Cas9 system in the brown planthopper Nilaparvata lugens, a serious insect pest of rice. Surprisingly, homozygous NlugGr23a mutant (NlugGr23a-/-) males were sterile but their sperm were motile and morphologically normal. DAPI staining of mutant sperm inseminated eggs showed that most of NlugGr23a-/- sperm failed to fertilize eggs, even if they were capable of entering into the egg as a result of their arrested development prior to male pronucleus formation. Immunohistochemistry demonstrated the expression of NlugGr23a in testis. Moreover, prior mating by NlugGr23a-/- males suppressed female fertility. To our knowledge, it is the first report that a chemoreceptor is implicated in male sterility and provides a potential molecular target for genetic pest control alternatives.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Drosophila
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Hemípteros
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Infertilidade Masculina
Tipo de estudo:
Etiology_studies
Limite:
Animals
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Female
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Humans
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Male
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article