Two mutations at KRT74 and EDAR synergistically drive the fine-wool production in Chinese sheep.
J Adv Res
; 57: 1-13, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-37137429
ABSTRACT
INTRODUCTION:
Fine-wool sheep are the most common breed used by the wool industry worldwide. Fine-wool sheep have over a three-fold higher follicle density and a 50% smaller fiber diameter than coarse-wool sheep.OBJECTIVES:
This study aims to clarify the underlying genetic basis for the denser and finer wool phenotype in fine-wool breeds.METHOD:
Whole-genome sequences of 140 samples, Ovine HD630K SNP array data of 385 samples, including fine, semi-fine, and coarse wool sheep, as well as skin transcriptomes of nine samples were integrated for genomic selection signature analysis.RESULTS:
Two loci at keratin 74 (KRT74) and ectodysplasin receptor (EDAR) were revealed. Fine-scale analysis in 250 fine/semi-fine and 198 coarse wool sheep narrowed this association to one C/A missense variant of KRT74 (OAR3133,486,008, P = 1.02E-67) and one T/C SNP in the regulatory region upstream of EDAR (OAR361,927,840, P = 2.50E-43). Cellular over-expression and ovine skin section staining assays confirmed that C-KRT74 activated the KRT74 protein and specifically enlarged cell size at the Huxley's layer of the inner root sheath (P < 0.01). This structure enhancement shapes the growing hair shaft into the finer wool than the wild type. Luciferase assays validated that the C-to-T mutation upregulated EDAR mRNA expression via a newly created SOX2 binding site and potentially led to the formation of more hair placodes.CONCLUSIONS:
Two functional mutations driving finer and denser wool production were characterized and offered new targets for genetic breeding during wool sheep selection. This study not only provides a theoretical basis for future selection of fine wool sheep breeds but also contributes to improving the value of wool commodities.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lã
/
Mutação de Sentido Incorreto
/
Queratinas Tipo II
/
Receptor Edar
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article