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A Low-Volume, Parallel Copper-Bicinchoninic Acid (BCA) Assay for Glycoside Hydrolases.
Arnal, Gregory; Attia, Mohamed A; Asohan, Jathavan; Lei, Zhenhuan; Golisch, Benedikt; Brumer, Harry.
Afiliação
  • Arnal G; Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada.
  • Attia MA; Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada.
  • Asohan J; Department of Chemistry, University of British Columbia, Vancouver, BC, Canada.
  • Lei Z; Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada.
  • Golisch B; Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada.
  • Brumer H; Department of Chemistry, University of British Columbia, Vancouver, BC, Canada.
Methods Mol Biol ; 2657: 3-14, 2023.
Article em En | MEDLINE | ID: mdl-37149519
ABSTRACT
The quantitation of liberated reducing sugars by the copper-bicinchoninic acid (BCA) assay provides a highly sensitive method for the measurement of glycoside hydrolase (GH) activity, particularly on soluble polysaccharide substrates. Here we describe a straightforward method adapted to low-volume polymerase chain reaction (PCR) tubes that enables the rapid, parallel determination of GH kinetics in applications ranging from initial activity screening and assay optimization to precise Michaelis-Menten analysis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quinolinas / Glicosídeo Hidrolases Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quinolinas / Glicosídeo Hidrolases Idioma: En Ano de publicação: 2023 Tipo de documento: Article