Long-Read Metagenomics and CAZyme Discovery.
Methods Mol Biol
; 2657: 253-284, 2023.
Article
em En
| MEDLINE
| ID: mdl-37149537
ABSTRACT
Microorganisms play a primary role in regulating biogeochemical cycles and are a valuable source of enzymes that have biotechnological applications, such as carbohydrate-active enzymes (CAZymes). However, the inability to culture the majority of microorganisms that exist in natural ecosystems restricts access to potentially novel bacteria and beneficial CAZymes. While commonplace molecular-based culture-independent methods such as metagenomics enable researchers to study microbial communities directly from environmental samples, recent progress in long-read sequencing technologies are advancing the field. We outline key methodological stages that are required as well as describe specific protocols that are currently used for long-read metagenomic projects dedicated to CAZyme discovery.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Metagenômica
/
Microbiota
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article