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A novel BRET-based assay to investigate binding and residence time of unmodified ligands to the human lysosomal ion channel TRPML1 in intact cells.
Cunha, Micael R; Catta-Preta, Carolina M C; Takarada, Jéssica E; Moreira, Gabriela A; Massirer, Katlin B; Couñago, Rafael M.
Afiliação
  • Cunha MR; Center of Medicinal Chemistry (CQMED), Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas, Campinas, Brazil. Electronic address: micaelrc@unicamp.br.
  • Catta-Preta CMC; Center of Medicinal Chemistry (CQMED), Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas, Campinas, Brazil.
  • Takarada JE; Center of Medicinal Chemistry (CQMED), Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas, Campinas, Brazil.
  • Moreira GA; Center of Medicinal Chemistry (CQMED), Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas, Campinas, Brazil.
  • Massirer KB; Center of Medicinal Chemistry (CQMED), Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas, Campinas, Brazil. Electronic address: kmassire@unicamp.br.
  • Couñago RM; Center of Medicinal Chemistry (CQMED), Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas, Campinas, Brazil; Structural Genomics Consortium and Division of Chemical Biology and Medicinal Chemistry, UNC Eshelman School of Pharmacy, University of North Carolina, Chape
J Biol Chem ; 299(6): 104807, 2023 06.
Article em En | MEDLINE | ID: mdl-37172730
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Canais de Potencial de Receptor Transitório / Técnicas de Transferência de Energia por Ressonância de Bioluminescência Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Canais de Potencial de Receptor Transitório / Técnicas de Transferência de Energia por Ressonância de Bioluminescência Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article