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MegaPro, a clinically translatable nanoparticle for in vivo tracking of stem cell implants in pig cartilage defects.
Suryadevara, Vidyani; Hajipour, Mohammad Javad; Adams, Lisa C; Aissaoui, Nour Mary; Rashidi, Ali; Kiru, Louise; Theruvath, Ashok J; Huang, Ching-Hsin; Maruyama, Masahiro; Tsubosaka, Masanori; Lyons, Jennifer K; Wu, Wei Emma; Roudi, Raheleh; Goodman, Stuart B; Daldrup-Link, Heike E.
Afiliação
  • Suryadevara V; Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
  • Hajipour MJ; Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
  • Adams LC; Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
  • Aissaoui NM; Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
  • Rashidi A; Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
  • Kiru L; Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
  • Theruvath AJ; Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
  • Huang CH; Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
  • Maruyama M; Department of Orthopedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Tsubosaka M; Department of Orthopedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Lyons JK; Department of Veterinary Medicine, Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, CA, USA.
  • Wu WE; Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
  • Roudi R; Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
  • Goodman SB; Department of Orthopedic Surgery, Stanford University School of Medicine, Stanford, CA, USA.
  • Daldrup-Link HE; Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
Theranostics ; 13(8): 2710-2720, 2023.
Article em En | MEDLINE | ID: mdl-37215574
ABSTRACT
Rationale Efficient labeling methods for mesenchymal stem cells (MSCs) are crucial for tracking and understanding their behavior in regenerative medicine applications, particularly in cartilage defects. MegaPro nanoparticles have emerged as a potential alternative to ferumoxytol nanoparticles for this purpose.

Methods:

In this study, we employed mechanoporation to develop an efficient labeling method for MSCs using MegaPro nanoparticles and compared their effectiveness with ferumoxytol nanoparticles in tracking MSCs and chondrogenic pellets. Pig MSCs were labeled with both nanoparticles using a custom-made microfluidic device, and their characteristics were analyzed using various imaging and spectroscopy techniques. The viability and differentiation capacity of labeled MSCs were also assessed. Labeled MSCs and chondrogenic pellets were implanted into pig knee joints and monitored using MRI and histological analysis.

Results:

MegaPro-labeled MSCs demonstrated shorter T2 relaxation times, higher iron content, and greater nanoparticle uptake compared to ferumoxytol-labeled MSCs, without significantly affecting their viability and differentiation capacity. Post-implantation, MegaPro-labeled MSCs and chondrogenic pellets displayed a strong hypointense signal on MRI with considerably shorter T2* relaxation times compared to adjacent cartilage. The hypointense signal of both MegaPro- and ferumoxytol-labeled chondrogenic pellets decreased over time. Histological evaluations showed regenerated defect areas and proteoglycan formation with no significant differences between the labeled groups.

Conclusion:

Our study demonstrates that mechanoporation with MegaPro nanoparticles enables efficient MSC labeling without affecting viability or differentiation. MegaPro-labeled cells show enhanced MRI tracking compared to ferumoxytol-labeled cells, emphasizing their potential in clinical stem cell therapies for cartilage defects.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Cartilagens / Transplante de Células-Tronco Mesenquimais / Nanopartículas Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Cartilagens / Transplante de Células-Tronco Mesenquimais / Nanopartículas Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article