In vivo cross-linking-based affinity purification and mass spectrometry for targeting intracellular protein-protein interactions.

Zhong, Bowen; An, Yuxin; Gao, Hang; Zhao, Lili; Li, Xiao; Liang, Zhen; Zhang, Yukui; Zhao, Qun; Zhang, Lihua

Zhong, Bowen; An, Yuxin; Gao, Hang; Zhao, Lili; Li, Xiao; Liang, Zhen; Zhang, Yukui; Zhao, Qun; Zhang, Lihua.

Afiliação

Zhong B; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, Liaoning, 116023, China.
An Y; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, Liaoning, 116023, China; University of Chinese Academy of Sciences, Beijing, 100039, China.
Gao H; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, Liaoning, 116023, China; University of Chinese Academy of Sciences, Beijing, 100039, China.
Zhao L; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, Liaoning, 116023, China; University of Chinese Academy of Sciences, Beijing, 100039, China.
Li X; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, Liaoning, 116023, China.
Liang Z; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, Liaoning, 116023, China.
Zhang Y; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, Liaoning, 116023, China.
Zhao Q; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, Liaoning, 116023, China. Electronic address: zhaoqun@dicp.ac.cn.
Zhang L; CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, Liaoning, 116023, China. Electronic address: lihuazhang@dicp.ac.cn.

Anal Chim Acta ; 1265: 341273, 2023 Jul 18.

Article em En | MEDLINE | ID: mdl-37230567

Assuntos

Mapeamento de Interação de Proteínas; Proteínas; Mapeamento de Interação de Proteínas/métodos; Espectrometria de Massas/métodos; Cromatografia de Afinidade/métodos; Proteínas/metabolismo; Reagentes de Ligações Cruzadas

Palavras-chave

Affinity purification mass spectrometry; Chemical cleavable crosslinker; In vivo cross-linking; Interaction proteins; Interaction sites; MAPK3

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas / Mapeamento de Interação de Proteínas Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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