Repressing expression of difficult-to-express recombinant proteins during the selection process increases productivity of CHO stable pools.

Maltais, Jean-Sébastien; Lord-Dufour, Simon; Morasse, Audrey; Stuible, Matthew; Loignon, Martin; Durocher, Yves

Maltais, Jean-Sébastien; Lord-Dufour, Simon; Morasse, Audrey; Stuible, Matthew; Loignon, Martin; Durocher, Yves.

Afiliação

Maltais JS; Mammalian Cell Expression, Human Health Therapeutics Research Centre, National Research Council Canada, Montréal, Québec, Canada.
Lord-Dufour S; Mammalian Cell Expression, Human Health Therapeutics Research Centre, National Research Council Canada, Montréal, Québec, Canada.
Morasse A; Mammalian Cell Expression, Human Health Therapeutics Research Centre, National Research Council Canada, Montréal, Québec, Canada.
Stuible M; Mammalian Cell Expression, Human Health Therapeutics Research Centre, National Research Council Canada, Montréal, Québec, Canada.
Loignon M; Mammalian Cell Expression, Human Health Therapeutics Research Centre, National Research Council Canada, Montréal, Québec, Canada.
Durocher Y; Mammalian Cell Expression, Human Health Therapeutics Research Centre, National Research Council Canada, Montréal, Québec, Canada.

Biotechnol Bioeng ; 120(10): 2840-2852, 2023 10.

Article em En | MEDLINE | ID: mdl-37232536

Assuntos

Anticorpos Monoclonais; Citocinas; Cricetinae; Animais; Cricetulus; Células CHO; Proteínas Recombinantes/metabolismo

Palavras-chave

CHO cells; ER stress; cumate; difficult-to-express protein; inducible expression; productivity

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citocinas / Anticorpos Monoclonais Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google