Phalloidin Staining for F-Actin in Hepatic Stellate Cells.
Methods Mol Biol
; 2669: 55-66, 2023.
Article
em En
| MEDLINE
| ID: mdl-37247054
ABSTRACT
During the development of liver fibrosis, hepatic stellate cells undergo a transition from a quiescent phenotype into a proliferative, fibrogenic, and contractile, α-smooth muscle actin-positive myofibroblast. These cells acquire properties that are strongly associated with the reorganization of the actin cytoskeleton. Actin possesses a unique ability to polymerize into filamentous actin (F-actin) form its monomeric globular state (G-actin). F-actin can form robust actin bundles and cytoskeletal networks by interacting with a number of actin-binding proteins that provide important mechanical and structural support for a multitude of cellular processes including intracellular transport, cell motility, polarity, cell shape, gene regulation, and signal transduction. Therefore, stains with actin-specific antibodies and phalloidin conjugates for actin staining are widely used to visualize actin structures in myofibroblasts. Here we present an optimized protocol for F-actin staining for hepatic stellate cells using a fluorescent phalloidin.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Actinas
/
Células Estreladas do Fígado
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article