A ThT Derivative as Zn2+ Sensor Based on DNA G-quadruplex.
J Fluoresc
; 34(1): 353-358, 2024 Jan.
Article
em En
| MEDLINE
| ID: mdl-37256463
ABSTRACT
In this report, we developed a sensing strategy based on ThT-E (a ThT derivative) and DNA G-quadruplex for the label-free detection of Zn2+. In the absence of Zn2+, there was a fluorescence enhancement of ThT-E by interaction with human telomere sequence. On the addition of Zn2+, Zn2+ induced a more compact antiparallel G-quadruplex to release ThT-E, resulting in fluorescence quenching. The detection limit was 0.6996 µM, and the fluorescence intensity showed a good linear relationship with the concentration of Zn2+ in the range of 0-10 µM. This sensing strategy which only needs to mix two kinds of materials has the characteristics of label-feel, simple operation, short response time, economical and efficient.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Quadruplex G
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article