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Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation.
Stephens, Dominique C; Mungai, Margaret; Crabtree, Amber; Beasley, Heather K; Garza-Lopez, Edgar; Vang, Larry; Neikirk, Kit; Vue, Zer; Vue, Neng; Marshall, Andrea G; Turner, Kyrin; Shao, Jian-Qiang; Sarker, Bishnu; Murray, Sandra; Gaddy, Jennifer A; Davis, Jamaine; Damo, Steven M; Hinton, Antentor O.
Afiliação
  • Stephens DC; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Mungai M; Department of Life and Physical Sciences, Fisk University, Nashville, TN, 37232, USA.
  • Crabtree A; Department of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA.
  • Beasley HK; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Garza-Lopez E; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Vang L; Department of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA.
  • Neikirk K; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Vue Z; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Vue N; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Marshall AG; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Turner K; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Shao JQ; Department of Life and Physical Sciences, Fisk University, Nashville, TN, 37232, USA.
  • Sarker B; Central Microscopy Research Facility, University of Iowa, Iowa City, IA, 52242, USA.
  • Murray S; School of Applied Computational Sciences, Meharry Medical College, Nashville, TN, 37232, USA.
  • Gaddy JA; Department of Cell Biology, College of Medicine, University of Pittsburgh, Pittsburgh, TN, 15260, USA.
  • Davis J; Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
  • Damo SM; Tennessee Valley Healthcare Systems, U.S. Department of Veterans Affairs, Nashville, Tennessee, USA.
  • Hinton AO; Department of Biochemistry and Cancer Biology. Meharry Medical College, Nashville, TN, USA.
bioRxiv ; 2023 Aug 19.
Article em En | MEDLINE | ID: mdl-37292961
ABSTRACT
Isolation of skeletal muscles allows for the exploration of many complex diseases. Fibroblasts and myoblast play important roles in skeletal muscle morphology and function. However, skeletal muscles are complex and made up of many cellular populations and validation of these populations is highly important. Therefore, in this article, we discuss a comprehensive method to isolate mice skeletal muscle, create satellite cells for tissue culture, and use immunofluorescence to validate our approach.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article