Nitrous Oxide-Induced Metaphase Arrest: A Technique for Somatic Chromosome Analysis.
Methods Mol Biol
; 2672: 129-139, 2023.
Article
em En
| MEDLINE
| ID: mdl-37335472
ABSTRACT
Procedures to arrest metaphase chromosomes are used for determining chromosome numbers, chromosomal aberrations, and natural chromosome variation, as well as chromosome sorting. Here is described a technique of nitrous oxide gas treatment of freshly harvested root tips that is highly effective at producing an excellent mitotic index together with well-spread chromosomes. The details of the treatment and equipment used are provided. The metaphase spreads can be used directly for determining chromosome numbers or for in situ hybridization to reveal chromosomal features.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aberrações Cromossômicas
/
Óxido Nitroso
Limite:
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article