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Dynamic in situ growth of bonded-phase silica nanospheres on silica capillary inner walls for open-tubular liquid chromatography.
Ahmed, Mohamed Adel; Ghiasvand, Alireza; Quirino, Joselito P.
Afiliação
  • Ahmed MA; Australian Centre for Research On Separation Science (ACROSS), School of Natural Sciences, University of Tasmania, Hobart, TAS, 7001, Australia.
  • Ghiasvand A; Australian Centre for Research On Separation Science (ACROSS), School of Natural Sciences, University of Tasmania, Hobart, TAS, 7001, Australia. alireza.ghiasvand@utas.edu.au.
  • Quirino JP; Department of Analytical Chemistry, Lorestan University, Khoramabad, Iran. alireza.ghiasvand@utas.edu.au.
Anal Bioanal Chem ; 415(20): 4923-4934, 2023 Aug.
Article em En | MEDLINE | ID: mdl-37351669
ABSTRACT
Silica nanospheres (SNS) were grown on the inner walls of silica capillaries through a dynamic in situ nucleation process to prepare a highly porous and large accessible surface area substrate. The SNS were then functionalized with octadecyl (C18), 3-aminopropyltriethoxysilane (APTES), beta-cyclodextrin (ß-CD), and amino groups to develop robust and efficient chromatographic stationary phases. The modified silica capillaries were exploited for open-tubular liquid chromatography (OT-LC) and open-tubular capillary electrochromatography (OT-CEC) applications. The prepared stationary phases were compared to conventional capillaries in terms of separation performance. The synthesis process was optimized, and the bonded-phase stationary phases were characterized by the electron microscopy technique. The effects of different solvents, additives, and functional groups on the geometry and chromatographic resolving power of the SNS were envisaged. The capillaries modified with octadecyl groups were evaluated for the separation of non-steroidal anti-inflammatory drugs, phenones, alkenylbenzenes, and enantiomers of chlorophenoxy herbicides. As an application instance, an SNS-C18-coated capillary was utilized for the separation of alkenylbenzenes from clove extract and protein digest medium, through OT-LC and OT-CEC techniques, respectively. The ß-CD functionalized capillary was applied for the OT-CEC separation of a dichlorprop racemic mixture.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article