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Localization Pattern of Dispatched Homolog 2 (DISP2) in the Central and Enteric Nervous System.
Heimke, Marvin; Richter, Florian; Heinze, Tillmann; Kunke, Madlen; Wedel, Thilo; Böttner, Martina; Egberts, Jan-Hendrik; Lucius, Ralph; Cossais, François.
Afiliação
  • Heimke M; Institute of Anatomy, Kiel University, Olshausenstrasse 40, 24098, Kiel, Germany.
  • Richter F; Department of General, Thoracic, Transplantation and Pediatric Surgery, University Hospital Schleswig-Holstein, Kiel University, Kiel, Germany.
  • Heinze T; Institute of Anatomy, Kiel University, Olshausenstrasse 40, 24098, Kiel, Germany.
  • Kunke M; Institute of Anatomy, Kiel University, Olshausenstrasse 40, 24098, Kiel, Germany.
  • Wedel T; Institute of Anatomy, Kiel University, Olshausenstrasse 40, 24098, Kiel, Germany.
  • Böttner M; Institute of Anatomy, Kiel University, Olshausenstrasse 40, 24098, Kiel, Germany.
  • Egberts JH; Department of Surgery, Israelite Hospital, Hamburg, Germany.
  • Lucius R; Institute of Anatomy, Kiel University, Olshausenstrasse 40, 24098, Kiel, Germany.
  • Cossais F; Institute of Anatomy, Kiel University, Olshausenstrasse 40, 24098, Kiel, Germany. f.cossais@anat.uni-kiel.de.
J Mol Neurosci ; 73(7-8): 539-548, 2023 Aug.
Article em En | MEDLINE | ID: mdl-37369878
ABSTRACT
Dispatched homolog (DISP) proteins have been implicated in the regulation of hedgehog signaling during embryologic development. Although DISP2 has recently been associated with neuronal development and control of cognitive functions, its localization pattern in the mammalian central and peripheral nervous system has not yet been investigated. In this study, the Disp2 expression profile was assessed in human tissues from publicly available transcriptomic datasets. The DISP2 localization pattern was further characterized in the human and rat central nervous system (CNS), as well as within the colonic enteric nervous system (ENS) using dual-label immunohistochemistry. Colocalization of DISP2 with neuronal and glial markers was additionally analyzed in murine primary ENS culture. At transcriptomic level, DISP2 expression was predominant in neuronal cell types of the CNS and ENS. DISP2 immunoreactivity was mainly located within PGP9.5-positive neurons rather than in S100-positive glial cells throughout the nervous system. Investigation of human and rat brain tissues, colonic specimens, and murine ENS primary cultures revealed that DISP2 was located in neuronal cell somata, as well as along neuronal processes both in the human and murine CNS and ENS. Our results indicate that DISP2 is prominently localized within neuronal cells of the CNS and ENS and support putative functions of DISP2 in these tissues.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sistema Nervoso Entérico / Proteínas Hedgehog Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sistema Nervoso Entérico / Proteínas Hedgehog Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article