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Development and Application of Real-Time PCR-Based Screening for Identification of Omicron SARS-CoV-2 Variant Sublineages.
Esman, Anna; Dubodelov, Dmitry; Khafizov, Kamil; Kotov, Ivan; Roev, German; Golubeva, Anna; Gasanov, Gasan; Korabelnikova, Marina; Turashev, Askar; Cherkashin, Evgeniy; Mironov, Konstantin; Cherkashina, Anna; Akimkin, Vasily.
Afiliação
  • Esman A; Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia.
  • Dubodelov D; Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia.
  • Khafizov K; Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia.
  • Kotov I; Moscow Institute of Physics and Technology, National Research University, 115184 Dolgoprudny, Russia.
  • Roev G; Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia.
  • Golubeva A; Moscow Institute of Physics and Technology, National Research University, 115184 Dolgoprudny, Russia.
  • Gasanov G; Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia.
  • Korabelnikova M; Moscow Institute of Physics and Technology, National Research University, 115184 Dolgoprudny, Russia.
  • Turashev A; Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia.
  • Cherkashin E; Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia.
  • Mironov K; Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia.
  • Cherkashina A; Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia.
  • Akimkin V; Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing Russian Federation, 3a Novogireevskaya Str., 111123 Moscow, Russia.
Genes (Basel) ; 14(6)2023 06 02.
Article em En | MEDLINE | ID: mdl-37372398
ABSTRACT
The Omicron strain is currently the main dominant variant of SARS-CoV-2, with a large number of sublineages. In this article, we present our experience in tracing it in Russia using molecular diagnostic methods. For this purpose, different approaches were used; for example, we developed multiprimer panels for RT-PCR and Sanger and NGS sequencing methods. For the centralized collection and analysis of samples, the VGARus database was developed, which currently includes more than 300,000 viral sequences.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: COVID-19 Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: COVID-19 Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article