Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification.

Imai, Hirotatsu; Utsumi, Daisuke; Torihara, Hidetsugu; Takahashi, Kenzo; Kuroyanagi, Hidehito; Yamashita, Akio

Imai, Hirotatsu; Utsumi, Daisuke; Torihara, Hidetsugu; Takahashi, Kenzo; Kuroyanagi, Hidehito; Yamashita, Akio.

Afiliação

Imai H; Department of Investigative Medicine, Graduate School of Medicine, University of the Ryukyus, Nishihara-cho, Okinawa 903-0215, Japan.
Utsumi D; Department of Dermatology, Graduate School of Medicine, University of the Ryukyus, Nishihara-cho, Okinawa 903-0215, Japan.
Torihara H; Department of Biochemistry, Graduate School of Medicine, University of the Ryukyus, Nishihara-cho, Okinawa 903-0215, Japan.
Takahashi K; Department of Dermatology, Graduate School of Medicine, University of the Ryukyus, Nishihara-cho, Okinawa 903-0215, Japan.
Kuroyanagi H; Department of Biochemistry, Graduate School of Medicine, University of the Ryukyus, Nishihara-cho, Okinawa 903-0215, Japan.
Yamashita A; Department of Investigative Medicine, Graduate School of Medicine, University of the Ryukyus, Nishihara-cho, Okinawa 903-0215, Japan.

Nucleic Acids Res ; 51(14): e76, 2023 08 11.

Article em En | MEDLINE | ID: mdl-37378452

Assuntos

Técnicas Genéticas; Biossíntese de Proteínas; Tiouridina; Transcriptoma; Animais; Mamíferos/genética; Perfil de Ribossomos; Ribossomos/genética; Ribossomos/metabolismo; RNA/metabolismo; Regulação da Expressão Gênica

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tiouridina / Biossíntese de Proteínas / Técnicas Genéticas / Transcriptoma Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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