Protocol for in vitro assessment of human monocyte transendothelial migration using a high-throughput live cell imaging system.
STAR Protoc
; 4(3): 102388, 2023 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-37379221
ABSTRACT
In vitro modeling of the different steps of immune cell recruitment is essential to decipher the role of endothelial cells in this process. Here, we present a protocol for the assessment of human monocyte transendothelial migration using a live cell imaging system. We describe steps for culture of fluorescent monocytic THP-1 cells and chemotaxis plate preparation with HUVEC monolayers. We then detail real-time analysis using the IncuCyte® S3 live-cell imaging system, image analysis, and assessment of transendothelial migration rates. For complete details on the use and execution of this protocol, please refer to Ladaigue et al.1.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Monócitos
/
Migração Transendotelial e Transepitelial
Limite:
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article