Creation of iPSC line NCHi004-A from a patient with down syndrome and congenital heart defects.
Stem Cell Res
; 71: 103156, 2023 09.
Article
em En
| MEDLINE
| ID: mdl-37393719
ABSTRACT
Down syndrome is a congenital disorder resulting from an extra full or partial chromosome 21, which is characterized by a spectrum of systemic developmental abnormalities, including those affecting the cardiovascular system. Here, we generated an iPSC line from peripheral blood mononuclear cells of a male adolescent with Down syndrome-associated congenital heart defects through Sendai virus-mediated transfection of 4 Yamanaka factors. This line exhibited normal morphology, expressed pluripotency markers, trisomy 21 karyotype, and could be differentiated into three germ layers. This iPSC line can be used for studying cellular and developmental etiologies of congenital heart defects induced by aneuploidy of chromosome 21.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Síndrome de Down
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Células-Tronco Pluripotentes Induzidas
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Cardiopatias Congênitas
Limite:
Adolescent
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Humans
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Male
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article