[Construction of a dual fluorescent reporter system for tracing horizontal transfer of mcr-1-carrying plasmid].
Zhonghua Yu Fang Yi Xue Za Zhi
; 57(7): 1063-1067, 2023 Jul 06.
Article
em Zh
| MEDLINE
| ID: mdl-37400217
ABSTRACT
The green fluorescent reporter gene was inserted into the gene interval of polymyxin resistant mcr-1-carrying plasmid (pSH13G841) by homologous recombination of suicide plasmid. At the same time, E. coli J53 with red fluorescent reporter gene was constructed. Using the ability of spontaneous conjugation of drug resistant plasmid (pSH13G841), pSH13G841-GFP plasmid was transferred into J53 RFP bacteria to construct a double fluorescent labeled donor bacterium. The two light-emitting systems could stably and spontaneously express fluorescence without mutual interference. The dual fluorescence report system constructed can be used for visual tracing horizontal transfer of mcr-1-carrying plasmid, the subsequent model can study the colonization, transfer and prognosis of drug-resistant bacteria/drug-resistant genes mcr-1 by using mouse in vivo imaging technology.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Escherichia coli
/
Escherichia coli
Limite:
Animals
/
Humans
Idioma:
Zh
Ano de publicação:
2023
Tipo de documento:
Article