Prokaryotic Argonautes for in vivo biotechnology and molecular diagnostics.
Trends Biotechnol
; 42(1): 61-73, 2024 01.
Article
em En
| MEDLINE
| ID: mdl-37451948
ABSTRACT
Prokaryotic Argonautes (pAgos) are an emerging class of programmable endonucleases that are believed to be more flexible than existing CRISPR-Cas systems and have significant potential for biotechnology. Current applications of pAgos include a myriad of molecular diagnostics and in vitro DNA assembly tools. However, efforts have historically been centered on thermophilic pAgo variants. To enable in vivo biotechnological applications such as gene editing, focus has shifted to pAgos from mesophilic organisms. We discuss what is known of pAgos, how they are being developed for various applications, and strategies to overcome current challenges to in vivo applications in prokaryotes and eukaryotes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Procarióticas
/
Patologia Molecular
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article