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Formation and Replacement of Bone and Tooth Mineralized Tissues in Green Iguanas (Iguana iguana) Revealed by In-Vivo Fluorescence Marking.
Green, Daniel R; Winkler, Daniela E; Leichliter, Jennifer N; Harms, Gregory S; Hatt, Jean-Michel; Clauss, Marcus; Tütken, Thomas.
Afiliação
  • Green DR; Lamont-Doherty Earth Observatory, Climate School, Columbia University, 2910 Broadway Level A, New York, NY 10025, USA.
  • Winkler DE; Applied and Analytical Palaeontology, Institute of Geosciences, Johannes Gutenberg University, J.-J.-Becher-Weg 21, 55128 Mainz, Germany.
  • Leichliter JN; Zoological Institute, Christian-Albrechts-Universität zu Kiel, Am Botanischen Garten 1-9, 24118 Kiel, Germany.
  • Harms GS; Applied and Analytical Palaeontology, Institute of Geosciences, Johannes Gutenberg University, J.-J.-Becher-Weg 21, 55128 Mainz, Germany.
  • Hatt JM; Emmy Noether Group for Hominin Meat Consumption, Max Planck Institute for Chemistry, 55128 Mainz, Germany.
  • Clauss M; Imaging Core Facility, University Medical Center Mainz, Langenbeckstraße 1, 55131 Mainz, Germany.
  • Tütken T; Departments of Biology and Earth Systems Science and Mathematics, Physics and Computer Science, WIlkes University, Wilkes-Barre, PA 18766, USA.
Integr Comp Biol ; 63(3): 515-529, 2023 09 15.
Article em En | MEDLINE | ID: mdl-37475667
ABSTRACT
Hard tissue formation patterns and rates reveal details of animal physiology, life history, and environment, but are understudied in reptiles. Here, we use fluorescence labels delivered in vivo and laser confocal scanning microscopy to study tooth and bone formation in a managed group of green iguanas (Iguana iguana, Linné 1758) kept for 1.5 years under experimentally controlled conditions and undergoing several dietary switches. We constrain rates of tooth elongation, which we observe to be slow when enamel is initially deposited (c. 9 µm/day), but then increases exponentially in the dentin root, reaching c. 55 µm/day or more after crown completion. We further constrain the total timing of tooth formation to ∼40-60 days, and observe highly variable timings of tooth resorption onset and replacement. Fluorescent labels clearly indicate cohorts of teeth recruited within Zahnreihen replacement waves, with faster sequential tooth recruitment and greater wave sizes posteriorly, where each wave initiates. Fluorescence further reveals enamel maturation after initial deposition. Rates of hard tissue formation in long bones range from 0.4 to 3.4 µm/day, correlating with animal weight gain and cortical bone recording the entire history of the experiment. We suggest additional labeling experiments to study hard tissue formation patterns in other reptiles, and propose strategies for chemical analyses of hard tissues in order to extract temporal information about past environments, behaviors, and diets from reptilian fossils throughout the Phanerozoic.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dente / Iguanas Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dente / Iguanas Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article