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In vitro propagation of infectious hypodermal hematopoietic necrosis virus (Penaeus stylirostris penstyldensovirus) in PmLyO-Sf9 cells.
Dominic, Dominic Vithayathil Diya; Sajeevan, Thavarool Puthiyedathu; Ameer, Ahna; Muneer, Abdulkhader; Eldho, Linu; Anoop, Bhaskaran Sathyabhama; Jayesh, Puthumana; Joseph, Valsamma; Philip, Rosamma; Singh, Isaac Sarojini Bright.
Afiliação
  • Dominic DVD; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, India.
  • Sajeevan TP; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, India.
  • Ameer A; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, India.
  • Muneer A; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, India.
  • Eldho L; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, India.
  • Anoop BS; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, India.
  • Jayesh P; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, India.
  • Joseph V; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, India.
  • Philip R; Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Kochi, India.
  • Singh ISB; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, India. isbsingh@gmail.com.
Arch Virol ; 168(8): 211, 2023 Jul 24.
Article em En | MEDLINE | ID: mdl-37486432
ABSTRACT
Infectious hypodermal hematopoietic necrosis virus (IHHNV/PstDVI) was isolated and propagated in the hybrid shrimp-insect cell line PmLyO-Sf9. A few hours after inoculation with an infected tissue extract or virus suspension, cytopathic changes could be observed in the cell line, including clustering, enlargement, syncytium formation, granulation, vacuole formation, tapering, irregularities in the plasma membrane with extended tails, detachment, cell death, and accumulation of cellular debris. Expression of viral genes, the presence of virions, and cytological changes observed using transmission electron microscopy suggested replication of the virus in these cells. The virus was purified by ultracentrifugation, negatively stained, and examined using an electron microscope, and the purified virus was found to be infectious both in vitro and in vivo. This development opens avenues for the study of the basic molecular mechanism of IHHNV infection, pathogenesis, and replication, which is much needed for developing an antiviral strategy in aquaculture.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Densovirinae / Vírus da Necrose Hematopoética Infecciosa / Penaeidae Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Densovirinae / Vírus da Necrose Hematopoética Infecciosa / Penaeidae Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article