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Pinoresinol rescues developmental phenotypes of Arabidopsis phenylpropanoid mutants overexpressing FERULATE 5-HYDROXYLASE.
Muro-Villanueva, Fabiola; Pysh, Leonard D; Kim, Hoon; Bouse, Tyler; Ralph, John; Luo, Zhiwei; Cooper, Bruce R; Jannasch, Amber S; Zhang, Zeyu; Gu, Chong; Chapple, Clint.
Afiliação
  • Muro-Villanueva F; Department of Biochemistry, Purdue University, West Lafayette, IN 47907.
  • Pysh LD; Center for Plant Biology, Purdue University, West Lafayette, IN 47907.
  • Kim H; Department of Biology, Roanoke College, Salem, VA 24153.
  • Bouse T; US Department of Energy's Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Madison, WI 53726.
  • Ralph J; Department of Biochemistry, Purdue University, West Lafayette, IN 47907.
  • Luo Z; US Department of Energy's Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Madison, WI 53726.
  • Cooper BR; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706.
  • Jannasch AS; Department of Biochemistry, Purdue University, West Lafayette, IN 47907.
  • Zhang Z; Center for Plant Biology, Purdue University, West Lafayette, IN 47907.
  • Gu C; Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907.
  • Chapple C; Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907.
Proc Natl Acad Sci U S A ; 120(31): e2216543120, 2023 08.
Article em En | MEDLINE | ID: mdl-37487096
Most phenylpropanoid pathway flux is directed toward the production of monolignols, but this pathway also generates multiple bioactive metabolites. The monolignols coniferyl and sinapyl alcohol polymerize to form guaiacyl (G) and syringyl (S) units in lignin, components that are characteristic of plant secondary cell walls. Lignin negatively impacts the saccharification potential of lignocellulosic biomass. Although manipulation of its content and composition through genetic engineering has reduced biomass recalcitrance, in some cases, these genetic manipulations lead to impaired growth. The reduced-growth phenotype is often attributed to poor water transport due to xylem collapse in low-lignin mutants, but alternative models suggest that it could be caused by the hyper- or hypoaccumulation of phenylpropanoid intermediates. In Arabidopsis thaliana, overexpression of FERULATE 5-HYDROXYLASE (F5H) shifts the normal G/S lignin ratio to nearly pure S lignin and does not result in substantial changes to plant growth. In contrast, when we overexpressed F5H in the low-lignin mutants cinnamyl dehydrogenase c and d (cadc cadd), cinnamoyl-CoA reductase 1, and reduced epidermal fluorescence 3, plant growth was severely compromised. In addition, cadc cadd plants overexpressing F5H exhibited defects in lateral root development. Exogenous coniferyl alcohol (CA) and its dimeric coupling product, pinoresinol, rescue these phenotypes. These data suggest that mutations in the phenylpropanoid pathway limit the biosynthesis of pinoresinol, and this effect is exacerbated by overexpression of F5H, which further draws down cellular pools of its precursor, CA. Overall, these genetic manipulations appear to restrict the synthesis of pinoresinol or a downstream metabolite that is necessary for plant growth.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Proteínas de Arabidopsis Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Proteínas de Arabidopsis Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article