Two-photon microendoscope for label-free imaging in stereotactic neurosurgery.
Biomed Opt Express
; 14(7): 3705-3725, 2023 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-37497482
ABSTRACT
We demonstrate a gradient refractive index (GRIN) microendoscope with an outer diameter of â¼1.2 mm and a length of â¼186 mm that can fit into a stereotactic surgical cannula. Two photon imaging at an excitation wavelength of 900â
nm showed a field of view of â¼180 microns and a lateral and axial resolution of 0.86 microns and 9.6 microns respectively. The microendoscope was tested by imaging autofluorescence and second harmonic generation (SHG) in label-free human brain tissue. Furthermore, preliminary image analysis indicates that image classification models can predict if an image is from the subthalamic nucleus or the surrounding tissue using conventional, bench-top two-photon autofluorescence.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article