Binding of cytosol 3H-17, beta-estradiol to cytosol receptors in the liver of female rats.

ABSTRACT

The article is a study of the specific binding of 3H-17, beta-estradiol to cytosol receptors in the liver of female, sexually mature albino rats sham-operated, ovariectomized and treated after the ovariectomy with 17-beta-estradiol (in doses of 20 micrograms/100 g body weight for 11 days). The binding of the labelled hormone in the liver cytosol isolated from ovariectomized rats is considerably reduced compared with the sham-operated animals. In the cytosol from the liver of the estradiol-treated rats the formation of a hormone-receptor complex is substantially increased compared with the ovariectomized animals. It is assumed that the formation of estradiol-receptor complexes in the liver depends on the concentration of the cytosol receptors, which is determined by the concentration of oestrogens in the blood.