Your browser doesn't support javascript.
loading
Fast, automated, continuous energy scans for experimental phasing at the BioMAX beamline.
Gorgisyan, Ishkhan; Bell, Paul; Cascella, Michele; Eguiraun, Mikel; Freitas, Áureo; Lidon-Simon, Julio; Nan, Jie; Takahashi, Carla; Tarawneh, Hamed; Ursby, Thomas; Gonzalez, Ana.
Afiliação
  • Gorgisyan I; MAX IV Laboratory, Fotongatan 2, 224 84 Lund, Sweden.
  • Bell P; MAX IV Laboratory, Fotongatan 2, 224 84 Lund, Sweden.
  • Cascella M; MAX IV Laboratory, Fotongatan 2, 224 84 Lund, Sweden.
  • Eguiraun M; MAX IV Laboratory, Fotongatan 2, 224 84 Lund, Sweden.
  • Freitas Á; MAX IV Laboratory, Fotongatan 2, 224 84 Lund, Sweden.
  • Lidon-Simon J; MAX IV Laboratory, Fotongatan 2, 224 84 Lund, Sweden.
  • Nan J; MAX IV Laboratory, Fotongatan 2, 224 84 Lund, Sweden.
  • Takahashi C; MAX IV Laboratory, Fotongatan 2, 224 84 Lund, Sweden.
  • Tarawneh H; MAX IV Laboratory, Fotongatan 2, 224 84 Lund, Sweden.
  • Ursby T; MAX IV Laboratory, Fotongatan 2, 224 84 Lund, Sweden.
  • Gonzalez A; MAX IV Laboratory, Fotongatan 2, 224 84 Lund, Sweden.
J Synchrotron Radiat ; 30(Pt 5): 885-894, 2023 Sep 01.
Article em En | MEDLINE | ID: mdl-37526994
In X-ray macromolecular crystallography (MX), single-wavelength anomalous dispersion (SAD) and multi-wavelength anomalous dispersion (MAD) techniques are commonly used for obtaining experimental phases. For an MX synchrotron beamline to support SAD and MAD techniques it is a prerequisite to have a reliable, fast and well automated energy scan routine. This work reports on a continuous energy scan procedure newly implemented at the BioMAX MX beamline at MAX IV Laboratory. The continuous energy scan is fully automated, capable of measuring accurate fluorescence counts over the absorption edge of interest while minimizing the sample exposure to X-rays, and is about a factor of five faster compared with a conventional step scan previously operational at BioMAX. The implementation of the continuous energy scan facilitates the prompt access to the anomalous scattering data, required for the SAD and MAD experiments.
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article