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Ursolic acid alleviates steroid-induced avascular necrosis of the femoral head in mouse by inhibiting apoptosis and rescuing osteogenic differentiation.
Liu, Qian; Wu, Yuyang; Li, Sisi; Yoon, Somy; Zhang, Jiaxin; Wang, Xiaoyi; Hu, Luoshuang; Su, Chenying; Zhang, Chunwu; Wu, Yungang.
Afiliação
  • Liu Q; Department of the Orthopedics of Traditional Chinese Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China; College of Pharmacy, Chonnam National University, Gwangju, Republic of Korea; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
  • Wu Y; School of the 1st Clinical Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
  • Li S; Department of Otolaryngology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
  • Yoon S; College of Pharmacy, Chonnam National University, Gwangju, Republic of Korea.
  • Zhang J; Department of the Orthopedics of Traditional Chinese Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
  • Wang X; Department of the Orthopedics of Traditional Chinese Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
  • Hu L; Department of the Orthopedics of Traditional Chinese Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
  • Su C; Department of the Orthopedics of Traditional Chinese Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
  • Zhang C; Department of the Orthopedics of Traditional Chinese Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. Electronic address: zcw6681@126.com.
  • Wu Y; Department of the Orthopedics of Traditional Chinese Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. Electronic address: zjwyg@126.com.
Toxicol Appl Pharmacol ; 475: 116649, 2023 09 15.
Article em En | MEDLINE | ID: mdl-37536651
ABSTRACT
Steroid-induced avascular necrosis of femoral head (SANFH) is a common disorder worldwide with high disability. Overdose of glucocorticoid (GC) is the most common non-traumatic cause of SANFH. Up until now, there are limited therapeutic strategies for curing SANFH, and the mechanisms underlying SANFH progression remain unclear. Nevertheless, Osteogenic dysfunction is considered to be one of the crucial pathobiological mechanisms in the development of SANFH, which involves mouse bone marrow mesenchymal stem cells (BMSCs) apoptosis and osteogenic differentiation disorder. Ursolic acid (UA), an important component of the Chinese medicine formula Yougui Yin, has a wide range of pharmacological properties such as anti-tumor, anti-inflammatory and bone remodeling. Due to the positive effect of Yougui Yin on bone remodeling, the purpose of this study was to investigate the effects of UA on dexamethasone (DEX)-induced SANFH in vitro and vivo. In vitro, we demonstrated that UA can promote mouse BMSCs proliferation and resist DEX-induced apoptosis by CCK8, Western blotting, TUNEL and so on. In addition, vitro experiments such as ALP and Alizarin red staining assay showed that UA had a beneficial effect on the osteogenic differentiation of mouse BMSCs. In vivo, the results of H&E staining, immunohistochemistry staining, Elisa and micro-CT analysis showed that UA had a bone repair-promoting effect in SANFH model. Moreover, the results of Western blot and TUNEL experiments showed that UA could delay the disease progression of SANFH in mice by inhibiting apoptosis. Overall, our study suggests that UA is a potential compound for the treatment of SANFH.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Necrose da Cabeça do Fêmur Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Necrose da Cabeça do Fêmur Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article