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Comprehensive Two-Dimensional Gas Chromatography with Peak Tracking for Screening of Constituent Biodegradation in Petroleum UVCB Substances.
Booth, Andy M; Sørensen, Lisbet; Brakstad, Odd G; Ribicic, Deni; Creese, Mari; Arey, J Samuel; Lyon, Delina Y; Redman, Aaron D; Martin-Aparicio, Alberto; Camenzuli, Louise; Wang, Neil; Gros, Jonas.
Afiliação
  • Booth AM; SINTEF Ocean, Trondheim NO-7465, Norway.
  • Sørensen L; SINTEF Ocean, Trondheim NO-7465, Norway.
  • Brakstad OG; SINTEF Ocean, Trondheim NO-7465, Norway.
  • Ribicic D; SINTEF Ocean, Trondheim NO-7465, Norway.
  • Creese M; SINTEF Ocean, Trondheim NO-7465, Norway.
  • Arey JS; Oleolytics LLC, Lebanon, New Jersey 08833, United States.
  • Lyon DY; Concawe, Brussels 1160, Belgium.
  • Redman AD; ExxonMobil Biomedical Sciences, Inc., Annandale, New Jersey 08801, United States.
  • Martin-Aparicio A; Concawe, Brussels 1160, Belgium.
  • Camenzuli L; Concawe, Brussels 1160, Belgium.
  • Wang N; ExxonMobil Petroleum & Chemical B.V., Machelen 1831, Belgium.
  • Gros J; Concawe, Brussels 1160, Belgium.
Environ Sci Technol ; 57(34): 12583-12593, 2023 08 29.
Article em En | MEDLINE | ID: mdl-37590158
ABSTRACT
Petroleum substances, as archetypical UVCBs (substances of unknown or variable composition, complex reaction products, or biological substances), pose a challenge for chemical risk assessment as they contain hundreds to thousands of individual constituents. It is particularly challenging to determine the biodegradability of petroleum substances since each constituent behaves differently. Testing the whole substance provides an average biodegradation, but it would be effectively impossible to obtain all constituents and test them individually. To overcome this challenge, comprehensive two-dimensional gas chromatography (GC × GC) in combination with advanced data-handling algorithms was applied to track and calculate degradation half-times (DT50s) of individual constituents in two dispersed middle distillate gas oils in seawater. By tracking >1000 peaks (representing ∼53-54% of the total mass across the entire chromatographic area), known biodegradation patterns of oil constituents were confirmed and extended to include many hundreds not currently investigated by traditional one-dimensional GC methods. Approximately 95% of the total tracked peak mass biodegraded after 64 days. By tracking the microbial community evolution, a correlation between the presence of functional microbial communities and the observed progression of DT50s between chemical classes was demonstrated. This approach could be used to screen the persistence of GC × GC-amenable constituents of petroleum substance UVCBs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Petróleo Tipo de estudo: Diagnostic_studies / Risk_factors_studies / Screening_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Petróleo Tipo de estudo: Diagnostic_studies / Risk_factors_studies / Screening_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article