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Antimicrobial efficacy of chlorine agents against selected oral pathogens.
Almhöjd, Ulrica Scherdin; Lehrkinder, Anna; Roos-Jansåker, Ann-Marie; Lingström, Peter.
Afiliação
  • Almhöjd US; Department of Cariology, Institute of Odontology, Sahlgrenska Academy, University of Gothenburg, Box 450, SE-405 30, Gothenburg, Sweden. ulrica.almhojd@odontologi.gu.se.
  • Lehrkinder A; Department of Cariology, Institute of Odontology, Sahlgrenska Academy, University of Gothenburg, Box 450, SE-405 30, Gothenburg, Sweden.
  • Roos-Jansåker AM; Department of Periodontology, Faculty of Odontology, Malmö University, SE-205 06, Malmö, Sweden.
  • Lingström P; Department of Cariology, Institute of Odontology, Sahlgrenska Academy, University of Gothenburg, Box 450, SE-405 30, Gothenburg, Sweden.
Clin Oral Investig ; 27(9): 5695-5707, 2023 Sep.
Article em En | MEDLINE | ID: mdl-37606720
ABSTRACT

OBJECTIVES:

Method-dependent comparison of antimicrobial agents' efficacy against oral pathogens. MATERIALS AND

METHODS:

Several sodium hypochlorite solutions (NaOCl)-Perisolv®, Carisolv® and Dakin's solution-were equated with chlorhexidine (CHX) and hydrogen peroxide (H2O2) against ten oral micro-organisms related to caries and periodontitis using different minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) techniques. Agents were adjusted to the final 70 mmol/L concentration of active chlorine molecule.

RESULTS:

Apart from H2O2 and the amino acids of Perisolv®, all the agents revealed an antimicrobial effect. Agar diffusion test ranked CHX (p < 0.05) as the most effective against all ten specimens, followed by the NaOCl of Perisolv® and Dakin's solution. Correspondingly, in broth microdilution on agar, CHX was the most effective in eradicating micro-organisms at 0.03 mmol/L compared with 2.2 mmol/L of Dakin's solution. In contrast, the bactericidal concentration of Dakin's solution was the most effective at 0.2 mmol/L, (p < 0.001), followed by Perisolv® (2.14 mmol/L), CHX (2.38 mmol/L) and Carisolv® (3.33 mmol/L) after 5 and 10 min in broth dilution test. In live/dead analysis, 60-min exposure to a 2-fold concentration of agents resulted in two-log Aggregatibacter actinomycetemcomitans inhibition by CHX (35 mmol/L) whilst Streptococcus mutans was more susceptible, in 0.8 and 8.8 mmol/L, after 10 min to CHX and Dakin's respectively.

CONCLUSION:

Replacement of CHX with tested hypochlorite agents showed evident potential and promoted rapid antimicrobial effect. CLINICAL RELEVANCE Effective antimicrobial agents are crucial in controlling pathogen-induced oral infections increasing clinical possibilities to combat oral biofilms. Additionally, CHX substitution with hypochlorite agents could eliminate CHX's adverse effects.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cloro / Anti-Infecciosos Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cloro / Anti-Infecciosos Idioma: En Ano de publicação: 2023 Tipo de documento: Article