Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging.
STAR Protoc
; 4(3): 102538, 2023 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-37624700
ABSTRACT
Temporal and spatial regulation of gene expression is crucial for proper embryonic development. Infrared laser-evoked gene operator (IR-LEGO) can provide information for various developmental processes. Here, we present a protocol to locally express cxcl12a during zebrafish olfactory organ development1 using a combination of IR-LEGO and live imaging. We describe steps for implementing IR-LEGO, biological sample preparation, live imaging, data collection, and analysis. This protocol can be applied to virtually any genetically modified experimental organism.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peixe-Zebra
/
Luz
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article